| 天道笃行 |
2010-01-26 09:32 |
DIC显微镜资料（诺马斯基棱镜相关）
看见不少朋友在询问此类信息，这里简单说一下，并提供一篇文章，供大家参考。 %,[p[`NRYR
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DIC即微分干涉相衬显微镜，其全称是Differential and Interference Contrast . kA% "-$3
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其采用偏光原理，起偏镜产生偏光后，就利用到诺马斯基棱镜了，即Nomarski，其作用是分解光束，分出两束振动方向一样，等强度的垂直光束，相干面由Nomarski棱镜的参数而定。观察的图像会极有立体感，观察效果逼真。其特点是分辨率高，且能观察透明物体。 1Ydym2
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Differential interference contrast microscopy (DIC), also known as Nomarski Interference Contrast (NIC) or Nomarski microscopy, is an optical microscopy illumination technique used to enhance the contrast in unstained, transparent samples. DIC works on the principle of interferometry to gain information about the optical density of the sample, to see otherwise invisible features. A relatively complex lighting scheme produces an image with the object appearing black to white on a grey background. This image is similar to that obtained by phase contrast microscopy but without the bright diffraction halo. j=~c(
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DIC works by separating a polarised light source into two beams which take slightly different paths through the sample. Where the length of each optical path (i.e. the product of refractive index and geometric path length) differs, the beams interfere when they are recombined. This gives the appearance of a three-dimensional physical relief corresponding to the variation of optical density of the sample, emphasising lines and edges though not providing a topographically accurate image.
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